Evolution of cancer cell lines draws concern

first_imgCell lines form the backbone of cancer research. These individual groups of cells, typically collected from patients’ tumor samples and cultured to grow indefinitely in the laboratory, enable everything from basic genetic research to drug discovery.But while scientists have thought that individual cell lines remain genetically uniform even as they continue to grow and divide, they can in fact evolve in ways that dramatically change their responses to drugs, say researchers from the Broad Institute and collaborating institutions. This continuing evolution of cells within cell lines — potentially driven by the laboratory conditions in which they are grown — may help explain why different studies that use the same cell lines often have conflicting results.These findings, reported in Nature, suggest that scientists need to take extra care to ensure that cell line-based models of cancer accurately reflect the tumor they are studying. The research team has released an online tool, Cell STRAINER, to help scientists benchmark their models.“The main message here is not that cell lines and culture-based models are bad. Rather, you should know your model, and understand its properties and limitations,” said Broad founding core member and Cancer Program director Todd Golub, who was the study’s co-senior author, along with Broad associate member and Dana-Farber Cancer Institute (DFCI) adult neuro-oncologist Rameen Beroukhim. “Knowing requires a level of genetic and genomic characterization beyond what we usually think about. Skipping this sort of careful characterization is not an option.”Evolution in a lab dishResearchers have long recognized that cell lines do not perfectly mimic their tumors of origin. Still, they are considered representative and stable enough to provide accurate insights into cancer biology and drug response. In fact, every cancer treatment available today was at some point tested in these laboratory models.However, scientists often struggle to reproduce study results from cell lines. In addition, no one has yet undertaken a systematic effort to measure how and the extent to which the cells in cancer cell lines change genetically over time, and whether those changes affect drug responses.“You can find many examples in the literature pointing out inconsistencies in drug sensitivity data from cell-line experiments,” said Uri Ben-David, the study’s first author and a postdoctoral fellow in the Golub lab. “We wanted to look for signs of evolution and connect the dots between changes within lines and those inconsistencies.”The researchers first began to suspect that these cell lines evolve when they reanalyzed sequencing data from 106 lines housed in two large collections: the Broad-Novartis Cancer Cell Line Encyclopedia (CCLE) and the Sanger Institute’s Genomics of Drug Sensitivity in Cancer. These lines should be genetically identical in both collections, but the team found high levels of variability. For instance, 19 percent of the genetic mutations the team found were present in only one collection or the other.The team then ran deep molecular analyses on 27 and 23 strains, respectively, of two widely used cell lines: the estrogen receptor-positive breast cancer line MCF-7, and the lung cancer line A549. The analysis included whole-genome DNA sequencing, targeted DNA sequencing of nearly 450 genes commonly mutated in cancer, and bulk and single-cell RNA sequencing. Each of the strains represented a batch of cells from the line with a distinct history (e.g., different kinds of lab manipulation, different length of time in culture, different original source).Their data revealed striking genomic differences between strains, ranging from single base pair mutations to large-scale changes in genome structure (e.g., losses of entire chromosome arms) to major changes in gene expression — all indicating that the cell lines were neither as stable nor as identical as researchers thought. These genetic and expression differences also affected the strains’ growth rates, cell size and shape, and other traits.The team extended their findings by carrying out targeted sequencing on an additional 11 cancer cells lines representing a variety of tumor types (e.g., prostate, colon, liver, skin). Across the board, the team found extensive genetic variability within lines.What drives these changes? By conducting experiments with different laboratory conditions, the team found that even changing the kind of nutrient media in which a cell line is cultivated can give some cells within a strain a growth advantage over others, allowing genetically distinct populations to evolve. And by extracting single cells and growing them in culture, the team also found that the progeny of isolated single cells could spontaneously acquire new mutations — showing that new, genetically diverse populations of cells could arise within a strain from individually isolated cells.Strain variation affects drug sensitivityTo understand how these genetic changes might affect drug response, the team tested the 27 MCF-7 strains against 321 drugs. Indeed, any given drug’s ability to slow the growth of or kill a strain’s cells correlated with the strain’s genetic features and its expression of genes targeted by the drug.The findings complement those of another study led by Ben-David, Beroukhim, and Golub that examined patient-derived xenograft (PDX) models, which are generated by implanting tumor cells from a cancer patient into a mouse. In 2017, they reported in Nature Genetics that over time human cancer cells in PDX models lost characteristic genetic features seen in patients and gained new features not encountered in humans, changes that correlated with shifts in the models’ drug sensitivities.A change in perspective, and an opportunityTaken together, the findings highlight a need for scientists to understand how the cells they are using may have diverged genetically from their parent line, so as to make accurate comparisons across experiments.To that end, the study team has developed and will release an online tool, Cell STRAINER, to help researchers estimate how much a cell line strain they use has diverged genetically from reference samples in the Cancer Cell Line Encyclopedia collection.The team also notes that the cell lines’ capacity for evolution could actually provide an opportunity to study a variety of aspects of cancer biology and tumor evolution.“Because the strains have the same general genetic background, you could test the same compound on two strains and use their features to study the compound’s mechanism of action, or the influences of gene expression differences on sensitivity,” said Golub, who is also the Charles A. Dana Investigator in Human Cancer Genetics at DFCI. “Or you could investigate tumor heterogeneity and the selective pressures that cause strains to evolve.”Support for this study came from the National Cancer Institute, the Human Frontier Science Program, the Gray Matters Brain Cancer Foundation, the Koch Institute Dana-Farber/Harvard Cancer Center Bridge Project, BroadNext10, and other sources. Todd Golub is an investigator with the Howard Hughes Medical Institute.last_img read more

West End Star Joanna Riding on Singing Gary Barlow’s Music in The Girls & More

first_img View Comments ‘The Girls’ star Joanna Riding (Photo: John Swannell) Two-time Olivier Award winner Joanna Riding has staked her formidable reputation on such musical theater classics as Carousel and My Fair Lady, now she is in previews at the Phoenix Theatre for the West End debut of a brand new British musical, The Girls, adapted from 2003 hit British film comedy Calendar Girls. Telling the true story of a community of Yorkshire women of all shapes, sizes, and ages who bared all for a calendar for charity, the show and its creators—composer Gary Barlow (Finding Neverland) included—are clearly near to Riding’s heart, as the gifted performer explained to Broadway.com.What initially drew you to the role of Annie in The Girls?It has quite simply one of the best scripts I have ever read, and I knew from that moment that I had to be involved in it. Since then, it’s been through so many workshops but [co-star] Claire Moore and I have wanted to stay involved with it throughout, and we have. We’ve hung on to the piece by our fingernails and are still here because we believe in it as a fantastic piece of theater—and a very honest British piece of theater.In what way “honest”?To start with, I’ve never come across lyrics like these [the authors are Gary Barlow and Tim Firth, the second of whom co-wrote the film and is directing the stage show]. They’re brutally honest about how real people feel; they’re not lovely platitudes and sweeping statements. Instead, you’ve got what real people say and think in times of great tragedy. When you come see it, you’ll know what I mean.Is there room for a stage musical of this material given that the 2003 film already spawned a West End play in 2008?There is. I mean, sure, some people will say, “Why are you doing this again?” but Tim [Firth] always knew that it would be a musical, so he’s sort of done it back-to-front. He was always carrying songs in his head and wanted to go there and once Gary Barlow got involved, there seemed to be a sort of alchemy between them: these songs have happened very organically.What is it like to sing Gary Barlow’s music?It’s immensely singable! I’ve been involved with the show for more than three years and who knows how many workshops and my two songs have remained entirely intact from the first day. By now, I feel as if the songs are singing themselves—they’re that good! And it helps that Gary is a lovely chap—so generous.Have you had to tone up physically given the nudity required for this story about a group of Yorkshire women who bare all for a calendar to raise money to help fight cancer?It’s been the reverse, really. I’ve always been very sporty and only have to look at a gym and I put muscle on, so as I get older I’ve gotten wirier instead of softer. My problem now is that I have absolutely no bosom!Is there the concern that some of the men in the audience might be expecting a sort of old-fashioned nudie musical?What we’ve become aware of on this already out of town is the wave of love that audiences feel towards us. It’s not about “look at my body,” it’s about celebrating womanhood and the audience’s love and gratitude towards a bunch of middle-aged women going, “Sod it, let’s be brave and do something we would never normally have done because John [the dead husband of Riding’s character, Annie] would have wanted it.”Does the production subvert people’s expectations?In a way. In fact, there’s nothing hugely on show; some of the women are more covered than they would be in a bikini. But there’s no doubt that we as a cast can feel the roller-coaster ride that the audience is on. People are laughing, then crying, then roaring again at what in effect is a great celebration of life and courage and family.If you’ve seen the film and then the play of the same name, will the musical in any way surprise you?The great thing is that Tim is very much a man who understands theater as well as film, so he has presented this very differently. In our version, you get more time to know the families and the menfolk and to get to know another generation, so that by getting to know the girls’ families, we give more of a damn, so that by the time the calendar does get done, you’re really rooting for them. This feels very much like a fresh version of their story, which is maybe why they’ve tampered with the title: it’s very much a new telling. What’s your relationship with Angela Baker, the real-life inspiration for your character, Annie?Where we could, we’ve met the women we’re playing and have heard their stories, which at times were tough to hear because of the deep emotion involved. But when people come up to me afterward and address me as if I were the character, I have to remind them that they’re confusing me with Annie. I haven’t been through what she’s been through, but people find the show so cathartic that they sort of assume I have.Did you present the material to the real-life women directly?Yes, we did a performance for the original calendar girls and their friends and family and the local community, but the show has continued on a journey since then. In a way, we’ve had such a long, luxurious time to play with the material that it can be dangerous because you can’t see the forest for the trees. That’s why it feels good to be onstage here in London: we need an audience back.Are there other classic musical theater roles you have your eye on, having been so remarkable as Julie Jordan and Eliza Doolittle among other parts?I do and Imelda Staunton is systematically destroying whatever thoughts I’ve had [laughs]! Every time I go to see her, I think, “She’s done it again! No one can do that role better,” whether it’s Miss Adelaide or whatever, so it’s going to take a long time to get her out of my head.Does it amaze you that the astounding Nicholas Hytner-directed National Theatre Carousel, with you as Julie and Janie Dee as Carrie, was almost 25 years ago?We were just talking about that yesterday, and I was such a greenhorn when I came to do that: I came out of nowhere and had done very little and all of a sudden it put me on the map. What Nick [Hytner] taught me was to be a lot less busy and to find a stillness that I am capable of, so it was probably the best acting lesson I ever had. Less is more where Jo Riding is concerned, so thank you, Nick. And it doesn’t feel like 25 years ago!Given the current musical climate in London, how do you think this show will fare?I think if we have the reaction [in London] that we had when we did it up north [in Leeds and Manchester], I think it will go down a storm and that people will respond very positively. The creative team has written an extraordinary piece but you just never know. There’s always that magical ingredient—almost a mystical ingredient—that can make a show fly, or not.How long is your contract?Twelve months and I want every one of those months and more. I haven’t invested in this for so long for it to disappear; I pray it goes the distance.last_img read more

Nassau Exec Mangano, Wife and Oyster Bay Supervisor Indicted, Feds Say

first_imgSign up for our COVID-19 newsletter to stay up-to-date on the latest coronavirus news throughout New York Nassau County Executive Ed Mangano, his wife, Linda, and Oyster Bay Town Supervisor John Venditto have been indicted on federal charges that will be unsealed Thursday, prosecutors said.Multiple New York news outlets quoting anonymous sources reported Wednesday that Mangano, a two-term Republican from Bethpage, would be charged in connection with the investigation into a Syosset restaurateur who’s been indicted for fraud and bribing a Town of Oyster Bay official. “County Executive Mangano has the highest integrity, has done nothing wrong, has not been contacted and the accusations are preposterous,” Mangano’s spokesman, Brian Nevin, told the Press Wednesday night. After prosecutors confirmed the indictment Thursday morning, Nevin directed calls to Mangano’s criminal defense attorney, Kevin Keating.Long Island state Senators Jack Martins, Carl Marcellino and Kemp Hannon, holding a press conference outside the Nassau County Legislature in Mineola Thursday morning, called on Mangano and Venditto to resign for the good of Nassau County and urged legislators and Oyster Bay Town Board to immediately begin the transition process to replace the indicted elected officials and ensure a smooth transition to new leadership. “The public process has to continue,” Martins said, adding that it’s important government work continue “undistracted.”“This is a sad day. It caught all of us by surprise,” said Marcellino, adding both must resign because “the people come first.”Hannon echoed their sentiment and said there should be “zero tolerance” for corruption.Nassau Republican Chair Joe Mondello did not return a request for comment.Nassau County Democratic Committee Chair Jay Jacobs released the following statement regarding the arrests:“This is a sad day for Nassau County and a sad day for Ed Mangano, John Venditto and their families. While we are adversaries in politics, I take no comfort or pleasure in what has happened today. If these allegations are true, they will represent a great widespread betrayal of the voters’ trust. Regardless of the legal outcome, there can no longer be any doubt that ‘business as usual’ in Nassau County politics and government must come to an end. The public’s demand for honest, credible and effective government requires it.”WNBC New York broke the story, which the other New York metro newspapers later reported, citing unnamed sources suggesting that the charges have to do with favors from Harendra Singh, who was arrested last year. It’s unclear if the reported probe has anything to do with prior revelations in the county.Chief Deputy Nassau County Executive Rob Walker said last year that federal investigators were probing his acceptance of donations from county contractors to Mangano’s re-election campaign. Walker testified as much while on the stand at the corruption trial of ex-New York State Senate Majority Leader Dean Skelos (R-Rockville Centre), who was convicted of fraud and other charges for, among other things, using his power to help secure a $12 million Nassau contract for a company that paid his son, Adam Skelos, who was also found guilty at trial. Both the father and son are appealing their convictions.Federal corruption investigations on Long Island of late haven’t been limited to Nassau, either. Across the county line, James Burke, the former Suffolk County chief of police, pleaded guilty in February to beating a suspect and orchestrating a cover up. He is scheduled to be sentenced Nov. 2.Former Suffolk County Conservative Party Chairman Ed Walsh was convicted in March of theft and fraud for golfing, gambling and politicking while on the clock at his job as a county corrections lieutenant. He is appealing his conviction and scheduled to be sentenced Nov. 18.Both the Burke and Walsh cases have reportedly spawned a federal investigation into the office of Suffolk County District Attorney Thomas Spota, which prompted Suffolk County Executive Steve Bellone to call on Spota to resign in May—a call Spota rebuffed.last_img read more

COVID-19: Participants of South Sulawesi religious mass gathering put in isolation

first_img“We’re in critical condition as the coronavirus has been spreading rapidly; therefore, we have to cancel the mass gathering to prevent the disease from spreading,” he said.The event committee’s chairman, Kahar, accepted the decision, saying that participants were about to leave the venue before the event ended.“We managed to hold the main event of praying together for our safety,” Kahar explained.Read also: Massive Bangladesh coronavirus prayer gathering sparks outcrySeparately, Makassar Immigration Office head Andi Pallawarukka said the 474 foreigners at the gathering had entered Indonesia legally.”They also underwent medical examinations at the airports […] and were declared healthy,” he said.Andi added that some of the foreign nationals had been in Indonesia for several months before the COVID-19 outbreak. Some arrived in Indonesia through Soekarno-Hatta International Airport in Tangerang, Banten and proceeded to Makassar using domestic flights. (aly)Topics : South Sulawesi health authorities put the participants of a suspended international Islamic event in Pakkato village, Bontomaranmu district, Gowa regency into isolation wards in several locations on Thursday.A total of 8,223 people were sent from the gathering venue in waves. Based on The Jakarta Post’s observation, authorities transported 474 foreign nationals from several countries by using bus escorted by police and military personnel.The foreigners came from at least 12 countries, including Malaysia, Singapore, Brunei Darussalam, Saudi Arabia, India, the Netherlands, Thailand and the Philippines. They were placed in hotels owned by the provincial administration in Makassar. After the foreigners, authorities transported Indonesians from outside South Sulawesi to a haj dormitory in the provincial capital of Makassar. Then police and military personnel escorted congregation members from the province, who were immediately sent to their respective homes.Meanwhile, people from outside South Sulawesi were allowed to stay in the city and leave the province according to their trip schedules. They are to depart from Sultan Hasanuddin International Airport and the Soekarno-Hatta seaport in Makassar.Read also: How a 16,000-strong religious gathering led Malaysia to lockdownSouth Sulawesi Governor Nurdin Abdullah said the administration had provided facilities in the haj dormitory, including a large tent, a public kitchen and toilets.last_img read more

Two killed, hundreds displaced in Sukabumi flash flood, more rain to come

first_imgA flash flood on Monday has left at least two people dead, 20 others injured and hundreds more displaced in Sukabumi, West Java.The flooding occurred around 5 p.m. local time on Monday, after a torrential downpour in the three districts of Cicurug, Parung Kuda and Cidahu caused the Citarik and Cipeuncit rivers to overflow their banks. The floodwaters were reportedly 6 meters high in certain areas.Three people in Cicurug were swept away by the flood. A joint search-and-rescue team recovered the bodies of two people identified as Jeje, 58, and Hasyim, 70, on Tuesday morning. The third person, identified as 25-year-old Anang, is still missing. “Twelve houses were swept away by the flash flood and 85 houses were inundated. Several vehicles were also swept away,” emergency head Budi Budiman Wahyu of the West Java Disaster Mitigation Agency (BPBD) said on Monday. Budi added that the four villages of Cisaat, Pasawahan, Mekarsari, and Bangbayang on the southern slope of Mount Salak were heavily affected by the floods. The West Java BPBD had sent five personnel to help the Sukabumi BPBD in providing assistance to residents in flood hazard areas, including their evacuation.The Sukabumi BPBD said on Tuesday that at least 20 people had been injured during the flooding, while 210 families in Cicurug had fled their homes for safety.Authorities also said that the flooding had affected at least 306 houses and several public facilities, and severely damaged six houses and 10 bridges.The Meteorology, Climatology and Geophysics Agency (BMKG) has issued an early warning for West Java, with potential heavy rainfall accompanied by lightning and strong winds on Tuesday and Wednesday.Topics :last_img read more

Iloilo’s ‘Top 10’ drug suspect falls

first_imgThe estimated market value of the recovered items is P5,000. A complaint for violation of Republic Act 9165 or the ComprehensiveDangerous Drugs Act of 2002 will be filed against Domingo. The suspect yielded six heat-sealed transparent plastic sachetscontaining suspected shabu; P500 buy-bust money; two cellphones; and a lighter,a report from the IPPO stated. Ulyses Domingo alias “Todoy”, a 39-year-old resident of Sitio Balabag,Barangay Poblacion, San Rafael town, was arrested on Jan. 16. It is better for these targets to surrender peacefully than resist thearresting policemen and die, said Philippine National Police acting directorPolice Lieutenant General Archie Francisco Gamboa during a visit to the PRO-6on Christmas Eve. Of the 105 drug targets, 50 are in Negros Occidental, 32 in BacolodCity, 11 in Iloilo province, four in Capiz, three each in Antique and IloiloCity, and one each in Guimaras and in Aklan. This 2020 the Philippine National Police in Western Visayas aims toget the 105 remaining high-value drug targets it failed to neutralize in 2019.Included in these targets are elected public officials serving as drugprotectors.center_img The goal, said Gamboa, is to arrest and jail the drug traffickers./PN ILOILO – A buy-bust operation in Barangay Natividad, Barotac Viejotown resulted in the arrest of a man whom the Iloilo Police Provincial Office(IPPO) described as the “top 10” suspected drug personality in the province. The No. 1 target remains to be Ernesto Bolivar, a resident of BarangayGuinacas, Pototan, Iloilo who has been in hiding since 2017. Police Colonel Roland Vilela, IPPO director, said Domingo’s arrest wasa result of his men’s “relentless campaign” against illegal drugs. Police units across the region are hunting him down, said PoliceLieutenant Colonel Joem Malong, spokesperson of the Police Regional Office 6(PRO-6).  last_img read more

Brazilian govt backing Neymar in racism row

first_img Loading… “In the face of yet another case of racism that has come to the surface in sport, the ministry of women, the family and human rights publicly voices its solidarity with the player Neymar Jr,” the ministry said in a statement. “Racism is a crime.”Advertisement Read Also: West Brom complete signing of ex-Chelsea defender The Brazilian striker slapped Gonzalez on the back of the head near the end of Sunday’s match, sparking a mass brawl that ended with five players sent off, including Neymar. He now faces a potential multi-match suspension, as does Gonzalez, if league officials find Neymar’s accusation is true. FacebookTwitterWhatsAppEmail分享 The statement came after Brazilian President Jair Bolsonaro on Monday retweeted Neymar’s own version of events.center_img Brazil’s government sent its support to Neymar Tuesday after he was red-carded for a fight in a Ligue 1 match in France, condemning the “racism” that triggered the brawl. The Paris Saint-Germain star is facing punishment by the French league’s disciplinary commission after slapping defender Alvaro Gonzalez near the end of PSG’s 1-0 loss to Marseille Sunday, accusing the Spaniard of calling him a “monkey.” Gonzalez denies the accusation. But in Neymar’s native Brazil, the ministry responsible for human rights issues said it stood behind the world’s most expensive footballer. Promoted ContentLook At Something Beautiful That Wasn’t Made By A Human BeingThe Funniest Prankster Grandma And Her GrandsonWho Earns More Than Ronaldo?The Highest Paid Football Players In The WorldBest & Worst Celebrity Endorsed Games Ever Made13 kids at weddings who just don’t give a hootA Soviet Shot Put Thrower’s Record Hasn’t Been Beaten To This Day7 Black Hole Facts That Will Change Your View Of The UniverseBig Actors Who Started Off With A Part In A Soap Opera10 Awesome TV Series That Got Cancelled Way Too SoonA Hurricane Can Be As Powerful As 10 Atomic Bombs7 Universities In The World With The Highest Market Valuelast_img read more

EC’s Bryer Hall Finishes 2nd At Wrestling State Finals

first_imgEast Central Trojan Wrestler Bryer Hall captures the runner-up spot in the 152# weight class at The State Finals on Saturday Night at Bankers Life Fieldhouse.Indiana 152 # #3 Bryer (46-2) came up short against #2 Jonathon Kervin (47-2) of Floyd Central by the score of 9-7 for The State Championship Crown.Hall became only the second Trojan Wrestler to make it to The State Wrestling Championship Finals. Arik Wolf was the runner-up in 2004. Arik’s brother, Adam, is the Head Coach of The Trojans Wrestling team. Another Wolf brother, Andrew, placed third back in 1998.last_img

Kenny assessed after Tramore fall

first_img Dr Adrian McGoldrick, senior medical officer for the Irish Turf Club, said: “Karen Kenny has suffered a small traumatic subdural haemorrhage and is currently being assessed. “She was unconscious for about three minutes but she was semi-conscious when she left. “She was able to respond to us and she knew she was in Tramore.” Dr McGoldrick continued: “She is much more stable now than she was when leaving here (Tramore). “She will be observed overnight.” Press Association The 7lb claimer was knocked unconscious when she was unshipped from Our Pearl two furlongs from home in the opening mile-and-a-half maiden. Kenny was airlifted to Cork University Hospital and underwent a brain scan. center_img Karen Kenny has suffered a “small subdural haemorrhage” after the jockey fell at Tramore on Saturday evening. last_img read more

Cricket News India vs Australia, 1st T20I HIGHLIGHTS: Australia beat India by 3 wickets to take 1-0 lead in two-match T20I series

first_img 20:09 (IST) 18:16 (IST) 20:33 (IST) What will be the ideal playing XI for both sides? Will we see Mayank Markande making his debut today in Vizag? 19:13 (IST) Rohit Sharma takes the strike for the first delivery, and he has been joined with a new opening partner KL Rahul. This remains to be seen if the duo can give India a great start.  18:19 (IST) Facebook Twitter Whats app Linked In Virat Kohli tried to go down the ground to a beautiful tossed up delivery by Adam Zampa, but couldn’t clear the fence and departs for 24 runs off 17 deliveries.  21:50 (IST) Facebook Twitter Whats app Linked In After the first 10 overs, India lost two wickets in quick succession, putting Australia ahead in the game. Losing KL Rahul and Dinesh Karthik, Krunal Pandya further dented India’s batting order. From here, the road is only looking tougher. However, MS Dhoni is still at the crease, who is also India’s last hope. In the last five overs, India managed to score just 20 runs.  BREAKING! Mayank Markande, the young wristspinner, will make his debut for India in the Vizag game against Australia. Proud moment for the youngster, who impressed for Mumbai Indians. Peter Handscomb is also making his T20I debut for Australia. 21:55 (IST) Third run out of the game. This time it’s D Arcy Short who pays the price. Is there a twist in this game? Facebook Twitter Whats app Linked In New Delhi: In the nail-biting encounter, it was Australia who held their nerves to clinch a superlative victory. Australia wins the first game by 3 wickets to take a 1-0 lead in a two-match T20 series.***India started the innings on a quiet note, having been able to score just one of the first over. However, India upped the scoring rate smashing two boundaries of the second over.  In the third over, India lost their vice-captain, Rohit Sharma, as he tried to play a scoop to Jason Behrendorff and wasn’t able to time it to perfection. After first five overs, the game slightly tilts in favour of Australia with India managing to score 38 for 1 in 5 overs. (LIVE SCORECARD)In the next leg, KL Rahul and Virat Kohli put up a partnership of over 50 runs before Virat Kohli departed. His departure led Rishabh Pant to the crease, and India had left-right and combination at the crease for the first time. However, as soon as India was going ahead in the game, Behrendorff produced a brilliant piece of fielding effort to send Pant back to the pavilion. After the first half of Indian innings, India is 80 runs for the loss of 3 wickets. (LIVE SCORECARD)After the first 10 overs, India lost two wickets in quick succession, putting Australia ahead in the game. Losing KL Rahul and Dinesh Karthik, Krunal Pandya further dented India’s batting order. From here, the road is only looking tougher. However, MS Dhoni is still at the crease, who is also India’s last hope. In the last five overs, India managed to score just 20 runs. (LIVE SCORECARD)Nathan Coulter Nile has been the pick of the bowler picking three main wickets to haunt India’s batting. From being 80 runs for the loss of 2 wickets, India was reduced 109 runs for 7 wickets. With Ms Dhoni, the only proper batsman at the crease, India is struggling to rotate the strike and as Dhoni tries to deal in boundaries.  (LIVE SCORECARD)At the end of India’s innings, it wouldn’t be wrong to say that the men-in-yellow came with a proper plan to give India a tough time in the middle. Except for Rahul’s quick-fire 50, India doesn’t have much to be proud of. Having scored 126 runs in their quota of 20 overs, it will be interesting to see bowlers manage to replicate Aussie’s thoughts. (LIVE SCORECARD)In the second innings, Indian bowlers strike early in the innings, sending in-form Marcus Stoinis and skipper Aaron Finch back to the pavilion. The duo managed to have just 1 run between them. (LIVE SCORECARD)Chasing a small total, all Australia needed, was a decent start. However, there was a little confusion in the middle which eventually led to a runout. In the next over, Jasprit Bumrah sent Aaron Finch for a golden duck. His departure led Glenn Maxwell to the crease who has already smashed four boundaries in 9 balls so far. His power-packed innings has helped Australia to come back into the game. After 5 overs, Australia is 28 runs for the loss of two wickets. (LIVE SCORECARD)From being 5 for the loss of 2 wickets, Australians suddenly saw themselves 41 for the loss of 2 wickets at the end of the powerplay – Credit to Glenn Maxwell’s clean hitting. Skipper Kohli introduced spin from both end post that, which eventually slowed things a bit. With this, Australia is well in control, scoring 67 runs in 10 overs. (LIVE SCORECARD) For India, this series is all about finalising the team composition ahead of the World Cup. For Australia, these are the last 12 international games across formats in which they can fix upon a winning team as they look to enter the World Cup high on confidence. Facebook Twitter Whats app Linked In The encounter between two top quality sides is all set to start in less than an hour. With this, the two teams are looking to make their last minute plans ahead of the toss which is scheduled to happen at 18:30 pm (IST).  Indian bowlers strike early in the innings, sending in-form Marcus Stoinis and skipper Aaron Finch back to the pavilion. The duo managed to have just 1 run between them.  The last two Twenty20 International series between India and Australia has ended 1-1. Can India secure an outright win this time? India started the innings on a quiet note, having been able to score just one of the first over. However, India upped the scoring rate smashing two boundaries of the second over.  In the third over, India lost their vice-captain, Rohit Sharma, as he tried to play a scoop to Jason Behrendorff and wasn’t able to time it to perfection. After first five overs, the game slightly tilts in favour of Australia with India managing to score 38 for 1 in 5 overs.  16 needed off the last two overs. Can India clinch this one? Krunal Pandya picks his first wicket of the night to send Turner for 0. Australia has now lost half their side. India needs to continue the momentum. 21:26 (IST) Facebook Twitter Whats app Linked In Facebook Twitter Whats app Linked In Chasing a small total, all Australia needed, was a decent start. However, there was a little confusion in the middle which eventually led to a runout. In the next over, Jasprit Bumrah sent Aaron Finch for a golden duck. His departure led Glenn Maxwell to the crease who has already smashed four boundaries in 9 balls so far. His power-packed innings has helped Australia to come back into the game. After 5 overs, Australia is 28 runs for the loss of two wickets.    Virat Kohli is back. Jasprit Bumrah is back. The Indian team assumes a potent look. Without these two players, the team has still managed to do well. Nathan Coulter-Nile awarded as the man-of-the-match for his dazzling spell of 3/26 in 4 overs. 19:22 (IST) Facebook Twitter Whats app Linked In 22:20 (IST) From being 5 for the loss of 2 wickets, Australians suddenly saw themselves 41 for the loss of 2 wickets at the end of the powerplay – Credit to Glenn Maxwell’s clean hitting. Skipper Kohli introduced spin from both end post that, which eventually slowed things a bit. With this, Australia is well in control, scoring 67 runs in 10 overs.  Facebook Twitter Whats app Linked In After Rohit falling early in the innings, skipper Virat Kohli and in-form KL Rahul put up a partnership of 51 runs, to help India be in a solid situation.  19:47 (IST) 19:37 (IST) Facebook Twitter Whats app Linked In 22:34 (IST) Facebook Twitter Whats app Linked In 20:52 (IST) In a nail-biting encounter, it was Australia who held their nerves to clinch a superlative victory. Australia wins the first game by 3 wickets to take a 1-0 lead in a two-match T20 series. The two teams joined for their respective National anthems, followed by two-minute silence for the CRPF jawans killed in the Pulwama Terror Attack in the district of Jammu and Kashmir.  After starting off the innings on a quiet note, KL Rahul and Virat Kohli put up a partnership of over 50 runs before Virat Kohli departed. His departure led Rishabh Pant to the crease, and India had left-right and combination at the crease for the first time. However, as soon as India was going ahead in the game, Behrendorff produced a brilliant piece of fielding effort to send Pant back to the pavilion. After the first half of Indian innings, India is 80 runs for the loss of 3 wickets. 19:42 (IST) 15 required off last 9 deliveries. Unbelievable bowling by India.  Facebook Twitter Whats app Linked In Facebook Twitter Whats app Linked Incenter_img Facebook Twitter Whats app Linked In 22:04 (IST) 18:32 (IST) Nathan Coulter Nile has been the pick of the bowler picking three main wickets to haunt India’s batting. From being 80 runs for the loss of 2 wickets, India was reduced 109 runs for 7 wickets. With Ms Dhoni, the only proper batsman at the crease, India is struggling to rotate the strike and as Dhoni tries to deal in boundaries.  MILESTONE ALERT! 1st T20I: Virat Kohli hits 500 runs vs Australia. India 19:02 (IST) Facebook Twitter Whats app Linked In At the end of India’s innings, it wouldn’t be wrong to say that the men-in-yellow came with a proper plan to give India a tough time in the middle. Except for Rahul’s quick-fire 50, India doesn’t have much to be proud of. Having scored 126 runs in their quota of 20 overs, it will be interesting to see bowlers manage to replicate Aussie’s thoughts.  Facebook Twitter Whats app Linked In For Australia, Marcus Stoinis and D Arcy Short start off the proceedings.  Indis lose their first wicket in form of Rohit Sharma. He tried to play a scoop to Jason Behrendorff but wasn’t able to time it perfectly. Vice-captain departs for just 5.  Facebook Twitter Whats app Linked In Facebook Twitter Whats app Linked In 20:57 (IST) 18:57 (IST) Facebook Twitter Whats app Linked In Glenn Maxwell’s sixth T20 International fifty led India to be all at sea and at the end of thirteenth over and it looked as if they have already fallen. However, India’s wrist spinner Yuzvendra Chahal came to bowl the fourteenth over and sent Maxwell back to the dressing room. Post this, India has managed to low the scoring rate as Australia see themselves at 98 runs for the loss of three wickets after 15 overs.  For all the Latest Sports News News, Cricket News News, Download News Nation Android and iOS Mobile Apps. Facebook Twitter Whats app Linked In 21:46 (IST) 19:25 (IST) 17:20 (IST) Facebook Twitter Whats app Linked In Facebook Twitter Whats app Linked In Facebook Twitter Whats app Linked In The pitch has plenty of cracks and it will assist spin. India might play three spinners on this track. The team that wins the toss should bat first and set a target in the range of 160. Australia has chosen to bowl in the Twenty20 International against India in Vizag. Peter Handscomb makes his debut and will keep wickets. Virat Kohli has said he switched off during the break and did not watch much cricket.  17:14 (IST) So, Mayank Markande replaces Vijay Shankar. Umesh Yadav replaces Bhuvneshwar Kumar while KL Rahul is in place of Shikhar Dhawan. Facebook Twitter Whats app Linked In 21:10 (IST) Facebook Twitter Whats app Linked In 18:14 (IST) 17:51 (IST) Facebook Twitter Whats app Linked In 20:23 (IST) Facebook Twitter Whats app Linked In Hello and welcome to the first T20I between India and Australia from the ADA-VDCA stadium in Vizag. Australia have won only once in India and they head into the contest low on confidence. Virat Kohli’s side, on the other hand, are high on confidence having achieved success in Australia and New Zealand recently. Facebook Twitter Whats app Linked In Facebook Twitter Whats app Linked In 18:23 (IST) Facebook Twitter Whats app Linked In Facebook Twitter Whats app Linked In 22:00 (IST) Facebook Twitter Whats app Linked In 18:35 (IST) 17:35 (IST) Facebook Twitter Whats app Linked Inlast_img read more